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primescripttm reverse transcriptase reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm reverse transcriptase reagent kit
    Primescripttm Reverse Transcriptase Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 4907 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+reverse+transcriptase/PrimeScript+Reverse+Transcriptase/pm42133204-232-8-13
    Average 97 stars, based on 4907 article reviews
    primescripttm reverse transcriptase reagent kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Comprehensive genome-wide identification, structural analysis, and cold-responsive expression profiling of double B-box (DBB) transcription factors in melon (Cucumis melo L.).
    Article Snippet: .. Total RNA was extracted from melon leaves with the help of RNase inhibitor (Thermo Fisher Scientific, AM2696) and DNA/RNA-free water (Tiangen Biotech (Beijing) Co., Ltd., RT121), and then reverse transcribed using PrimeScriptTM reverse transcriptase (TaKaRa Bio Engineering (Dalian) Co., Ltd., 2680A). ..

    Article Title: A brain-persistent DDR2-degrading antibody reverses Alzheimer’s pathologies by restoring brain fluid dynamics and metabolic clearance
    Article Snippet: Movement trajectories were tracked using EthoVision XT 15 software (Noldus). .. Total RNA was isolated from mice brain tissues using EZ-press RNA Purification Kit (EZBioscience, B0004D), followed by cDNA synthesis with PrimeScriptTM Reverse Transcriptase (Takara, 2690S) according to manufacturer protocols. .. Quantitative real-time PCR was performed using 2 × SYBR Green qPCR Mix (EZBioscience, A0012).

    Article Title: Gel-free library preparation for next-generation RNA sequencing and small RNA quantification.
    Article Snippet: Excess Linker 1 not ligated to RNAs was then deadenylated using 5’-deadenylase (New England Biolabs) through incubation at 30 °C for 1 h and subsequently digested with RecJf exonuclease (New England Biolabs) through two 30-min incubations at 37 °C. .. Reverse transcription (RT) was initiated with a 2-min incubation at 80 °C to anneal the RT primers to the RNA template, followed by a 2-h incubation at 50 °C to synthesize the cDNA strand using the PrimeScriptTM reverse transcriptase (RTase; TaKaRa) and a 15-min incubation at 70 °C to deactivate the enzyme. .. After the synthesis of the cDNA strand, the RNA template was hydrolyzed using sodium hydroxide (Sigma-Aldrich) through incubation at 95 °C for 3 min, followed by immediate neutralization using hydrochloric acid (VWR).

    Article Title: Jasmonic Acid Signals Involved in Valsa Canker Resistance Caused by C2H2-Type Transcription Factor PbeSTOP2 in Pyrus betulifolia
    Article Snippet: .. First-strand cDNA was then synthesized from 1 μg of the DNase-treated RNA using PrimeScriptTM Reverse Transcriptase (Takara Bio Inc., Kusatsu, Japan) and oligo(dT) 18 primers in a 20-μL reaction volume. .. The reverse transcription was performed at 42 °C for 30 min, with subsequent enzyme inactivation at 85 °C for 5 s. qPCR was performed with SYBR ® Green Premix (Bio-Rad) on a CFX96 TouchTM system.

    Article Title: Extracellular vesicle-integrated core-shell nanofibers synergize multi-target healing mechanisms for accelerated repair of large-volume skin defects
    Article Snippet: .. Total RNA was isolated from 1 × 10 6 cells per sample using TRIzolTM Reagent (Thermo Fisher Scientific, USA), followed by complementary DNA synthesis with PrimeScriptTM Reverse Transcriptase (Takara, Japan). ..

    Article Title: Cell flocculation and phase-separation support macro-scale tissue slab construction in a scaffold-free manner
    Article Snippet: .. RNA concentration and purity were determined by A260 and A260/A280 ratio. cDNA was synthesized using PrimeScriptTM Reverse Transcriptase (Takara, CA. ..

    Article Title: Polystyrene nanoplastics induce hepatic steatosis by disrupting autophagic degradation of NCoR1 and suppressing PPARα-mediated fatty acid oxidation.
    Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS was synthesized using PrimeScriptTM reverse transcriptase (Takara RR037A, Japan) and oligo-dT primers under defined thermal cycling conditions. .. Quantitative PCR (qPCR) was carried out using SYBR Green-based detection (ABI A25742, USA) on a Bio-Rad MyiQ realtime PCR system.

    Article Title: Extracellular Vesicle-Integrated Core-Shell Nanofibers Synergize Multi-Target Healing Mechanisms for Accelerated Repair of Large-Volume Skin Defects
    Article Snippet: .. Total RNA was isolated from 1 × 106 cells per sample using TRIzolTM Reagent (Thermo Fisher Scientific, USA), followed by complementary DNA synthesis with PrimeScriptTM Reverse Transcriptase (Takara, Japan). ..

    Isolation:

    Article Title: A brain-persistent DDR2-degrading antibody reverses Alzheimer’s pathologies by restoring brain fluid dynamics and metabolic clearance
    Article Snippet: Movement trajectories were tracked using EthoVision XT 15 software (Noldus). .. Total RNA was isolated from mice brain tissues using EZ-press RNA Purification Kit (EZBioscience, B0004D), followed by cDNA synthesis with PrimeScriptTM Reverse Transcriptase (Takara, 2690S) according to manufacturer protocols. .. Quantitative real-time PCR was performed using 2 × SYBR Green qPCR Mix (EZBioscience, A0012).

    Article Title: Extracellular vesicle-integrated core-shell nanofibers synergize multi-target healing mechanisms for accelerated repair of large-volume skin defects
    Article Snippet: .. Total RNA was isolated from 1 × 10 6 cells per sample using TRIzolTM Reagent (Thermo Fisher Scientific, USA), followed by complementary DNA synthesis with PrimeScriptTM Reverse Transcriptase (Takara, Japan). ..

    Article Title: Extracellular Vesicle-Integrated Core-Shell Nanofibers Synergize Multi-Target Healing Mechanisms for Accelerated Repair of Large-Volume Skin Defects
    Article Snippet: .. Total RNA was isolated from 1 × 106 cells per sample using TRIzolTM Reagent (Thermo Fisher Scientific, USA), followed by complementary DNA synthesis with PrimeScriptTM Reverse Transcriptase (Takara, Japan). ..

    Purification:

    Article Title: A brain-persistent DDR2-degrading antibody reverses Alzheimer’s pathologies by restoring brain fluid dynamics and metabolic clearance
    Article Snippet: Movement trajectories were tracked using EthoVision XT 15 software (Noldus). .. Total RNA was isolated from mice brain tissues using EZ-press RNA Purification Kit (EZBioscience, B0004D), followed by cDNA synthesis with PrimeScriptTM Reverse Transcriptase (Takara, 2690S) according to manufacturer protocols. .. Quantitative real-time PCR was performed using 2 × SYBR Green qPCR Mix (EZBioscience, A0012).

    cDNA Synthesis:

    Article Title: A brain-persistent DDR2-degrading antibody reverses Alzheimer’s pathologies by restoring brain fluid dynamics and metabolic clearance
    Article Snippet: Movement trajectories were tracked using EthoVision XT 15 software (Noldus). .. Total RNA was isolated from mice brain tissues using EZ-press RNA Purification Kit (EZBioscience, B0004D), followed by cDNA synthesis with PrimeScriptTM Reverse Transcriptase (Takara, 2690S) according to manufacturer protocols. .. Quantitative real-time PCR was performed using 2 × SYBR Green qPCR Mix (EZBioscience, A0012).

    Incubation:

    Article Title: Gel-free library preparation for next-generation RNA sequencing and small RNA quantification.
    Article Snippet: Excess Linker 1 not ligated to RNAs was then deadenylated using 5’-deadenylase (New England Biolabs) through incubation at 30 °C for 1 h and subsequently digested with RecJf exonuclease (New England Biolabs) through two 30-min incubations at 37 °C. .. Reverse transcription (RT) was initiated with a 2-min incubation at 80 °C to anneal the RT primers to the RNA template, followed by a 2-h incubation at 50 °C to synthesize the cDNA strand using the PrimeScriptTM reverse transcriptase (RTase; TaKaRa) and a 15-min incubation at 70 °C to deactivate the enzyme. .. After the synthesis of the cDNA strand, the RNA template was hydrolyzed using sodium hydroxide (Sigma-Aldrich) through incubation at 95 °C for 3 min, followed by immediate neutralization using hydrochloric acid (VWR).

    Synthesized:

    Article Title: Jasmonic Acid Signals Involved in Valsa Canker Resistance Caused by C2H2-Type Transcription Factor PbeSTOP2 in Pyrus betulifolia
    Article Snippet: .. First-strand cDNA was then synthesized from 1 μg of the DNase-treated RNA using PrimeScriptTM Reverse Transcriptase (Takara Bio Inc., Kusatsu, Japan) and oligo(dT) 18 primers in a 20-μL reaction volume. .. The reverse transcription was performed at 42 °C for 30 min, with subsequent enzyme inactivation at 85 °C for 5 s. qPCR was performed with SYBR ® Green Premix (Bio-Rad) on a CFX96 TouchTM system.

    Article Title: Cell flocculation and phase-separation support macro-scale tissue slab construction in a scaffold-free manner
    Article Snippet: .. RNA concentration and purity were determined by A260 and A260/A280 ratio. cDNA was synthesized using PrimeScriptTM Reverse Transcriptase (Takara, CA. ..

    Article Title: Polystyrene nanoplastics induce hepatic steatosis by disrupting autophagic degradation of NCoR1 and suppressing PPARα-mediated fatty acid oxidation.
    Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS was synthesized using PrimeScriptTM reverse transcriptase (Takara RR037A, Japan) and oligo-dT primers under defined thermal cycling conditions. .. Quantitative PCR (qPCR) was carried out using SYBR Green-based detection (ABI A25742, USA) on a Bio-Rad MyiQ realtime PCR system.

    DNA Synthesis:

    Article Title: Extracellular vesicle-integrated core-shell nanofibers synergize multi-target healing mechanisms for accelerated repair of large-volume skin defects
    Article Snippet: .. Total RNA was isolated from 1 × 10 6 cells per sample using TRIzolTM Reagent (Thermo Fisher Scientific, USA), followed by complementary DNA synthesis with PrimeScriptTM Reverse Transcriptase (Takara, Japan). ..

    Article Title: Extracellular Vesicle-Integrated Core-Shell Nanofibers Synergize Multi-Target Healing Mechanisms for Accelerated Repair of Large-Volume Skin Defects
    Article Snippet: .. Total RNA was isolated from 1 × 106 cells per sample using TRIzolTM Reagent (Thermo Fisher Scientific, USA), followed by complementary DNA synthesis with PrimeScriptTM Reverse Transcriptase (Takara, Japan). ..

    Concentration Assay:

    Article Title: Cell flocculation and phase-separation support macro-scale tissue slab construction in a scaffold-free manner
    Article Snippet: .. RNA concentration and purity were determined by A260 and A260/A280 ratio. cDNA was synthesized using PrimeScriptTM Reverse Transcriptase (Takara, CA. ..



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